Using enzymatic modification, we incorporate an alkyne functional group at the C-terminus of our products. Azide-containing cargos can then be coupled to our proteins using standard click-chemistry methods.
Cell surface localization of CCL2 - 647nm (left) and CXCL12 LD 647nm (right) to MDCK cells transfected with ACKR1. Cells were incubated with labeled chemokine for 1 hour prior to imaging. Hoechst stain was used to stain DNA in the CCL2 image.