Protein Foundry’s recombinant chemokines are expressed in E. coli bacterial cultures using plasmid based vectors engineered to yield chemokines with native N-termini. A series of ion exchange and reversed phase high-performance liquid chromatography (RP-HPLC) steps are utilized to ensure that our recombinant chemokines maintain the highest level of purity and the native disulfide bond pairings found in chemokines extracted from their native sources. Purified products are subjected to a rigorous set of quality control standards that include:
The rigorous purification protocols and quality control standards employed at Protein Foundry ensure lot-to-lot consistency required for any successful research campaign.
* All recombinant proteins produced and sold by Protein Foundry, LLC are <0.01 EU per 1 µg of the protein as determined by the LAL method.