Description: Recombinant human CXCL3 is expressed in E. coli, refolded and purified to yield the mature form of protein that is secreted from cells after cleavage of the signal peptide. Human CXCL3 is a 73 amino acid ELR+ chemokine that belongs to the GRO isoform subset which activate the G protein-coupled receptors CXCR1/CXCR2. LPS-stimulated macrophages strongly express CXCL2 in addition to the constitutive expression by macrophages. Expression of CXCL3 induces monocyte arrest and is a potent neutrophil chemoattractant.
Amino Acid Sequence:
ASVVTELRCQ CLQTLQGIHL KNIQSVNVRS PGPHCAQTEV IATLKNGKKA CLNPASPMVQ KIIEKILNKG STN
Molecular Weight:
7861.3 Daltons
Fusion Tag:
N/A
Special Characteristic(s):
Purity:
Purity assessed by HPLC, Mass Spectrometry, and NMR*
Source:
Human protein expressed in E. coli
Physical Form:
Lyophilized
Solubility:
Freely soluble in water **
Storage Conditions:
Product Form
Temperature
Storage Time
-20C to -80C
12 months
4C
6 months
RT
1 month
Additional comments or descriptive information:
* All recombinant proteins produced and sold by Protein Foundry, LLC are endotoxin-free <0.01 EU/ug of protein and validated using RP-HPLC, 2D-NMR, and functional reporter assay where available.
** Avoid repeated freeze-thaw cycles after reconstitution
