Description: Recombinant human CXCL3 is expressed in E. coli, refolded and purified to yield the mature form of protein that is secreted from cells after cleavage of the signal peptide. Human CXCL3 is a 73 amino acid ELR+ chemokine that belongs to the GRO isoform subset which activate the G protein-coupled receptors CXCR1/CXCR2. LPS-stimulated macrophages strongly express CXCL2 in addition to the constitutive expression by macrophages. Expression of CXCL3 induces monocyte arrest and is a potent neutrophil chemoattractant.
Amino Acid Sequence:
ASVVTELRCQ CLQTLQGIHL KNIQSVNVRS PGPHCAQTEV IATLKNGKKA CLNPASPMVQ KIIEKILNKG STN
Purity assessed by HPLC, Mass Spectrometry, and NMR*
Human protein expressed in E. coli
Freely soluble in water **
-20C to -80C
Additional comments or descriptive information:
* All recombinant proteins produced and sold by Protein Foundry, LLC are endotoxin-free <0.01 EU/ug of protein and validated using RP-HPLC, 2D-NMR, and functional reporter assay where available.
** Avoid repeated freeze-thaw cycles after reconstitution