Human CCL28 is chemokine that is constitutively expressed by epithelial cells in diverse mucosal tissues and is known to attract a variety of immune cell types including T-cell subsets and eosinophils through activation of the G protein-coupled receptors CCR10 and CCR3. CCL28 contains an extended C-terminal domain consisting of ~ 40 flexible amino acids. In addition to the two disulfide bonds that are conserved throughout the chemokine family, two additional cysteine residues at positions 30 and 80 form a novel disulfide linkage between the folded chemokine domain and the flexible C-terminus. Human CCL28 is expressed as an immature polypeptide 127 amino acids in length. Two different mature N-terminal sequences have been described due to uncertainty in the site of cleavage by signal peptidase. CCL28(1-108) is described in the UniProt entry as the mature form and consists of residues 20-127 of the uncleaved expressed protein. CCL28(1-108) is biologically active but may exhibit lower potency as a CCR10 agonist than the shorter version [CCL28(4-108)] that lacks the three N-terminal residues (SEA).
| Purity|| Assessed by HPLC, Mass Spectrometry, and NMR |
| Source|| Human protein expressed in E. coli|
| Physical form|| Lyophilized powder|
| Solubility|| Freely Soluble in Water|
| Accession #|| |
| Endotoxin level|| <0.01 EU per 1µg of the protein by LAL method|